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| "Guitar Man" by Jerry Reed | |
| Bo (Seann William Scott) and Luke (Johnny Knoxville) drive along in their Dodge Charger delivering moonshine. 0:00 | UK US |
| "One Way Out" by The Allman Brothers Band | |
| The guys race along in the Charger getting chased by 2 guys shooting at them because Luke was caught fooling around with their daughter / sister. 0:04 | UK US |
| "Good Ol' Boys" by Waylon Jennings | |
| Title screen. The narrater tells us about the guys. They land the car after a big jump but almost hit a lorry. 0:06 | UK US |
| "Hey Yeah" by Jimmie Vaughan | |
| Bo and Luke enter the bar with Deputy Sheriff Enos and stand by the bar. 0:10 | UK US |
| "Better Watch Yourself" by Bo Diddley | |
| In the bar Daisy (Jessica Simpson) bends down to pick up a coin. Dil stares at her and cracks jokes when she comes over. 0:10 | UK US |
| "Roosevelt and Ira Lee (Night of the Mossacin)" by Tony Joe White | |
| Bo and Luke point at Billy Prickett and order beers. Bo takes him a drink and banters with him. 0:11 | UK US |
| "Call Me the Breeze" by Lynyrd Skynyrd | |
| Bo starts a fight with Dil and his friends in the bar. Luke and Daisy join in. 0:13 | UK US |
| "I Don't Do Windows" by Hank Cochran | |
| Sheriff Rosco gives Cooter a hard time in his shop and tells him that he'll be auctioning off the General Lee. 0:24 | UK US |
| "Busted in Baylor County" by Shooter Jennings | |
| Using Cooter's tow truck, Bo and Luke drag a safe out of Jefferson Hogg's office. 0:26 | UK US |
| "All Out of Love" by Air Supply | |
| Plays on the tow truck radio as Luke tries to free the safe from the pole. 0:27 | UK US |
| "Busted in Baylor County" by Shooter Jennings | |
| The safe breaks free from the pole with Luke hanging on for dear life. 0:28 | UK US |
| "My Wife Thinks You're Dead" by Junior Brown | |
| Jefferson Hogg cooks as Sheriff Rosco tells him that the Duke boys have stolen his safe. 0:30 | US |
| "Mississippi Queen" by Mountain | |
| Bo and Luke race past the police officers in their newly refurbished General Lee. Sheriff Rosco radios in that he's chasing the Duke boys but they find they have a flat tire. 0:38 | UK US |
| "The South's Gonna Do It" by The Charlie Daniels Band | |
| Bo and Luke sitting in traffic on the way to Atlanta. A lorry driver gives them encouragement. 0:41 | UK US |
| "Hillbilly Shoes" by Montgomery Gentry | |
| Just after the previous song a van driver gives them encouragement. 0:41 | UK US |
| "Burn It Off" by Blues Explosion | |
| Bo and Luke arrive at Atlanta University. 0:44 | US |
| "Rockin' Chair" by Gwen McCrae | |
| Bo and Luke open the door to a college room in the search for Katie. It's the wrong one and the girls tell them she's down the hall. 0:46 | UK US |
| "Do It (Til You're Satisfied)" by B.T. Express | |
| They open another door and again it's wrong. The girls are boxing and tell them Katie's down the hall. 0:46 | UK US |
| "Automatic" by Noiseshaper | |
| They open the third door to find a room of naked girls. This time they stay. 0:46 | UK US |
| "Oh What a Feeling" by Wailing Souls | |
| They leave the third room to find Katie in the hall. 0:47 | UK US |
| "Change My Mind" by The Blueskins | |
| The guys ride in the General Lee with Katie and Annette in the back. They get pulled over by the campus police. 0:49 | UK US |
| Bo and Luke get some trouble from black guys by the side of the road. 0:56 | |
| "La Grange" by ZZ Top | |
| The guys race from the cops in Atlanta. Luke gets out the map and tries to give directions. Instrumental. 1:03 | UK US |
| "If You Want Blood You Got It" by AC/DC | |
| After Bo finds out about Luke and Katie he refuses to look at the road then drives even more recklessly. 1:04 | |
| "Shoot to Thrill" by AC/DC | |
| The Duke boys are chased by police. Luke shoots flaming arrows at the police cars. 1:16 | |
| "Black Betty" by Ram Jam | |
| At the big rally. Instrumental. 1:20 | UK US |
| "Flirtin' with Disaster" by Molly Hatchet | |
| Bo arrives at the rally in the General Lee and is still being chased by the police. A news reporter reports from the rally. 1:22 | UK US |
| "Funk #49" by James Gang | |
| Luke appears driving Jefferson Hogg's car and rams police cars. Uncle Jesse throws flaming bottles of moonshine out the back at the police. 1:24 | UK US |
| "These Boots are Made for Walkin'" by Jessica Simpson | |
| Daisy approaches the police blockade to get the police's help. 1:27 | US |
| "Soul City" by Southern Culture on the Skids | |
| Back on the rally. Bo overtakes the other racers and catches up with Billy Prickett. 1:28 | US |
| "Good Ol' Boys" by Willie Nelson | |
| 1st song in end credits, Willie Nelson performs live. Outtakes. 1:37 | UK US |
| "These Boots are Made for Walkin'" by Jessica Simpson | |
| 2nd song in end credits. Originally by Nancy Sinatra. 1:40 | US |
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